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human dermal papilla cells hdpcs (PromoCell)

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PromoCell human dermal papilla cells hdpcs
Representative human dermal papilla cell spheroids formed using the hanging drop method. Top row: Here, 0.1 mg/mL Corning Rat Tail Collagen I was added to a 10 µL suspension of 3000 <t>HDPCs</t> <t>and</t> <t>cultured</t> for 24 h. Bottom row: No collagen was added to the cells. Scale bar = 100 µm.

Representative human dermal papilla cell spheroids formed using the hanging drop method. Top row: Here, 0.1 mg/mL Corning Rat Tail Collagen I was added to a 10 µL suspension of 3000 <t>HDPCs</t> <t>and</t> <t>cultured</t> for 24 h. Bottom row: No collagen was added to the cells. Scale bar = 100 µm.

Human Dermal Papilla Cells Hdpcs, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+dermal+papilla+cells+hdpcs/Human+Follicle+Dermal+Papilla+Cells/pmc12729662-39-0-5
Average 95 stars, based on 130 article reviews

human dermal papilla cells hdpcs - by Bioz Stars, 2026-09

95/100 stars

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1) Product Images from "A Novel Approach to Pattern Dermal Papilla Spheroids in Dermal–Epidermal Composites Using Non-Adherent Microwell Arrays"

Article Title: A Novel Approach to Pattern Dermal Papilla Spheroids in Dermal–Epidermal Composites Using Non-Adherent Microwell Arrays

Journal: Bioengineering

doi: 10.3390/bioengineering12121281

Figure Legend Snippet: Representative human dermal papilla cell spheroids formed using the hanging drop method. Top row: Here, 0.1 mg/mL Corning Rat Tail Collagen I was added to a 10 µL suspension of 3000 HDPCs and cultured for 24 h. Bottom row: No collagen was added to the cells. Scale bar = 100 µm.

Techniques Used: Suspension, Cell Culture

Singularized HDPC response to culture in egg crate-designed microwell arrays fabricated from different non-adherent substrate materials. Stereolithography 3D printed stamps with an egg crate microwell pattern were used to mold 2% agarose ( A ), 5% agarose ( B ), and PDMS ( C ) substrate materials. HDPCs at 3000 cells per microwell were added to the arrays, cultured for 24 h, and imaged using light microscopy. The softer 2% and 5% agarose hydrogel substrate materials did not result in spheroid formation but did effectively pattern the singularized HDPCs. The stiffer silicone elastomer PDMS microwell arrays did result in HDPC spheroid formation and patterning when seeded with singularized HDPCs. Scale bars = 200 µm.

Figure Legend Snippet: Singularized HDPC response to culture in egg crate-designed microwell arrays fabricated from different non-adherent substrate materials. Stereolithography 3D printed stamps with an egg crate microwell pattern were used to mold 2% agarose ( A ), 5% agarose ( B ), and PDMS ( C ) substrate materials. HDPCs at 3000 cells per microwell were added to the arrays, cultured for 24 h, and imaged using light microscopy. The softer 2% and 5% agarose hydrogel substrate materials did not result in spheroid formation but did effectively pattern the singularized HDPCs. The stiffer silicone elastomer PDMS microwell arrays did result in HDPC spheroid formation and patterning when seeded with singularized HDPCs. Scale bars = 200 µm.

Techniques Used: Cell Culture, Light Microscopy

Distance between HDPC spheroids over time. Quantification of the distance of patterned HDPC spheroids formed in PDMS microwell arrays (1 d), transferred to the collagen matrix dermal compartment (3 d), cultured in epidermalization media (5 d), and transitioned to the air–liquid interface. The average distance of patterned spheroids decreases as the collagen matrix contracts and is reorganized by HDPCs. Scale bars = 200 µm. Note that the 1 d image on the top left is the same image shown in C. Significance comparing microwell spheroid distance at 1 day to later timepoints is denoted at statistical levels: *** p < 0.001.

Figure Legend Snippet: Distance between HDPC spheroids over time. Quantification of the distance of patterned HDPC spheroids formed in PDMS microwell arrays (1 d), transferred to the collagen matrix dermal compartment (3 d), cultured in epidermalization media (5 d), and transitioned to the air–liquid interface. The average distance of patterned spheroids decreases as the collagen matrix contracts and is reorganized by HDPCs. Scale bars = 200 µm. Note that the 1 d image on the top left is the same image shown in C. Significance comparing microwell spheroid distance at 1 day to later timepoints is denoted at statistical levels: *** p < 0.001.

Techniques Used: Cell Culture

Cell Culture:

Article Title: Glycogen Phosphorylase Inhibitor Promotes Hair Growth via Protecting from Oxidative-Stress and Regulating Glycogen Breakdown in Human Hair follicles.
Article Snippet: Hair follicles are multi-compartmented small organs that growth cycle goes through anagen, catagen, and telogen phase.. The dermal portion of the hair follicle can be divided into two compartments, the dermal papilla and dermal sheath (Paus and Cotsarelis, 1999).. Human Dermal Papilla Cells (hDPCs) and Human Outer Root Sheath Cells (hORSCs) are considered key cells particularly involved in skin development and hair growth and maintenance (Rajendran et al., 2022).

Article Title: A Novel Approach to Pattern Dermal Papilla Spheroids in Dermal–Epidermal Composites Using Non-Adherent Microwell Arrays
Article Snippet: .. Human dermal papilla cells (HDPCs) (PromoCell, Heidelberg, Germany) were cultured in complete Follicle Dermal Papilla Cell Growth Medium (HDP-M) (PromoCell, Heidelberg, Germany) supplemented with 1% Penicillin–Streptomycin (Gibco, Grand Island, NY, USA), 0.1% Gentamicin (Gibco), and 0.2% Amphotericin (Gibco). ..

Article Title: Red Ginseng Extract Promotes the Hair Growth in Cultured Human Hair Follicles
Article Snippet: .. Human dermal papilla cells (hDPCs) were isolated as described previously, 15 cultured in Follicle Dermal Papilla Cell Media (PromoCell, Heidelberg, Germany; supplemented with 0.04 mL/mL fetal calf serum, 0.004 mL/mL bovine pituitary extract, 1 ng/mL basic fibroblast growth factor, and 5 μ g/mL insulin), and passaged one to three times before use in this study. ..

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Journal: Bioengineering

Article Title: A Novel Approach to Pattern Dermal Papilla Spheroids in Dermal–Epidermal Composites Using Non-Adherent Microwell Arrays

doi: 10.3390/bioengineering12121281

Figure Lengend Snippet: Representative human dermal papilla cell spheroids formed using the hanging drop method. Top row: Here, 0.1 mg/mL Corning Rat Tail Collagen I was added to a 10 µL suspension of 3000 HDPCs and cultured for 24 h. Bottom row: No collagen was added to the cells. Scale bar = 100 µm.

Article Snippet: Human dermal papilla cells (HDPCs) (PromoCell, Heidelberg, Germany) were cultured in complete Follicle Dermal Papilla Cell Growth Medium (HDP-M) (PromoCell, Heidelberg, Germany) supplemented with 1% Penicillin–Streptomycin (Gibco, Grand Island, NY, USA), 0.1% Gentamicin (Gibco), and 0.2% Amphotericin (Gibco).

Techniques: Suspension, Cell Culture

Journal: Bioengineering

Article Title: A Novel Approach to Pattern Dermal Papilla Spheroids in Dermal–Epidermal Composites Using Non-Adherent Microwell Arrays

doi: 10.3390/bioengineering12121281

Figure Lengend Snippet: Singularized HDPC response to culture in egg crate-designed microwell arrays fabricated from different non-adherent substrate materials. Stereolithography 3D printed stamps with an egg crate microwell pattern were used to mold 2% agarose ( A ), 5% agarose ( B ), and PDMS ( C ) substrate materials. HDPCs at 3000 cells per microwell were added to the arrays, cultured for 24 h, and imaged using light microscopy. The softer 2% and 5% agarose hydrogel substrate materials did not result in spheroid formation but did effectively pattern the singularized HDPCs. The stiffer silicone elastomer PDMS microwell arrays did result in HDPC spheroid formation and patterning when seeded with singularized HDPCs. Scale bars = 200 µm.

Techniques: Cell Culture, Light Microscopy

Journal: Bioengineering

Article Title: A Novel Approach to Pattern Dermal Papilla Spheroids in Dermal–Epidermal Composites Using Non-Adherent Microwell Arrays

doi: 10.3390/bioengineering12121281

Figure Lengend Snippet: Distance between HDPC spheroids over time. Quantification of the distance of patterned HDPC spheroids formed in PDMS microwell arrays (1 d), transferred to the collagen matrix dermal compartment (3 d), cultured in epidermalization media (5 d), and transitioned to the air–liquid interface. The average distance of patterned spheroids decreases as the collagen matrix contracts and is reorganized by HDPCs. Scale bars = 200 µm. Note that the 1 d image on the top left is the same image shown in C. Significance comparing microwell spheroid distance at 1 day to later timepoints is denoted at statistical levels: *** p < 0.001.

Techniques: Cell Culture

PDLLA filler restores cell-cycle activity and paracrine function in senescent hDPCs, thereby promoting keratin synthesis in senescent hHFKs. ( A ) Schematic overview of the hDPC experimental design. hDPCs (1 × 10 6 cells) were treated with H 2 O 2 (150 µM) for 1.5 h, cultured in fresh growth medium for 3 days, and subsequently treated with PDLLA filler (300 µg/mL). Cells and CM were harvested 2 days after PDLLA filler treatment. ( B – D ) Cell-cycle distribution of senescent hDPCs analyzed by flow cytometry using PI staining. Percentages of cells in the G0/G1 ( B ), S ( C ), and G2/M ( D ) phases are shown. ( E ) Proliferation of senescent hDPCs assessed by proliferation assay after PDLLA filler treatment and expressed as fold change relative to PBS-treated senescent controls. ( F ) IGF-1 secretion level in CM from senescent hDPCs was quantified by ELISA and expressed as fold change relative to PBS-treated controls. ( G ) Schematic overview of the hHFK experimental design. Senescent hHFKs were cultured with CM derived from PBS-treated or PDLLA filler-treated senescent hDPCs. ( H ) Proliferation of hHFKs assessed after exposure to CM from PBS-treated (CM PBS ) or PDLLA filler-treated hDPCs (CM PDLLA filler ). ( I ) Western blot analysis of pan-keratin expression in senescent hHFKs. Molecular weight markers are indicated; pan-keratin bands were expected at 46–58 kDa. GAPDH served as the loading control. ( J ) Quantitative densitometric analysis of pan-keratin protein levels shown in ( I ), normalized to GAPDH and expressed as fold change relative to CM PBS . Data are presented as mean ± standard deviation. Differences among groups were analyzed using the Kruskal-Wallis test, with Mann–Whitney U tests used for post hoc pairwise comparisons. *, p < 0.05 and **, p < 0.01 vs. PBS or CM PBS . CM, conditioned medium; d, days; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; h, hours; hDPCs, human dermal papilla cells; hHFKs, human hair follicular keratinocytes; H 2 O 2, hydrogen peroxide; IGF-1, Insulin-like growth factor-1; PBS, phosphate-buffered saline; PDLLA, poly-D,L-lactic acid; PI, propidium iodide.

Journal: International Journal of Molecular Sciences

Article Title: Poly-D,L-Lactic Acid Filler Restores Hair Thickness and Shine by Ameliorating Age-Associated Follicular Decline

doi: 10.3390/ijms27052098

Figure Lengend Snippet: PDLLA filler restores cell-cycle activity and paracrine function in senescent hDPCs, thereby promoting keratin synthesis in senescent hHFKs. ( A ) Schematic overview of the hDPC experimental design. hDPCs (1 × 10 6 cells) were treated with H 2 O 2 (150 µM) for 1.5 h, cultured in fresh growth medium for 3 days, and subsequently treated with PDLLA filler (300 µg/mL). Cells and CM were harvested 2 days after PDLLA filler treatment. ( B – D ) Cell-cycle distribution of senescent hDPCs analyzed by flow cytometry using PI staining. Percentages of cells in the G0/G1 ( B ), S ( C ), and G2/M ( D ) phases are shown. ( E ) Proliferation of senescent hDPCs assessed by proliferation assay after PDLLA filler treatment and expressed as fold change relative to PBS-treated senescent controls. ( F ) IGF-1 secretion level in CM from senescent hDPCs was quantified by ELISA and expressed as fold change relative to PBS-treated controls. ( G ) Schematic overview of the hHFK experimental design. Senescent hHFKs were cultured with CM derived from PBS-treated or PDLLA filler-treated senescent hDPCs. ( H ) Proliferation of hHFKs assessed after exposure to CM from PBS-treated (CM PBS ) or PDLLA filler-treated hDPCs (CM PDLLA filler ). ( I ) Western blot analysis of pan-keratin expression in senescent hHFKs. Molecular weight markers are indicated; pan-keratin bands were expected at 46–58 kDa. GAPDH served as the loading control. ( J ) Quantitative densitometric analysis of pan-keratin protein levels shown in ( I ), normalized to GAPDH and expressed as fold change relative to CM PBS . Data are presented as mean ± standard deviation. Differences among groups were analyzed using the Kruskal-Wallis test, with Mann–Whitney U tests used for post hoc pairwise comparisons. *, p < 0.05 and **, p < 0.01 vs. PBS or CM PBS . CM, conditioned medium; d, days; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; h, hours; hDPCs, human dermal papilla cells; hHFKs, human hair follicular keratinocytes; H 2 O 2, hydrogen peroxide; IGF-1, Insulin-like growth factor-1; PBS, phosphate-buffered saline; PDLLA, poly-D,L-lactic acid; PI, propidium iodide.

Article Snippet: Human DPCs (hDPCs) were purchased from PromoCell GmbH (Heidelberg, Germany) and cultured in Follicle Dermal Papilla Cell Growth Medium (PromoCell) supplemented with the provided growth supplement mix and 1% penicillin/streptomycin, in accordance with the manufacturer’s instructions.

Techniques: Activity Assay, Cell Culture, Flow Cytometry, Staining, Proliferation Assay, Enzyme-linked Immunosorbent Assay, Derivative Assay, Western Blot, Expressing, Molecular Weight, Control, Standard Deviation, MANN-WHITNEY, Saline

PDLLA filler restores dermal papilla cell proliferation and promotes HMK proliferation in the hair matrix, as well as hair shaft keratin formation, in vivo. ( A ) Schematic illustration indicating the dermal papilla region within the hair follicle, where DPCs are densely localized and were analyzed for proliferation. Darker shaded areas indicate analyzed regions (dermal papilla). ( B ) Representative immunofluorescence images showing PCNA (green) expression in the dermal papilla region of hair follicles from saline- and PDLLA filler-treated mice. Nuclei were counterstained with DAPI (blue). Dashed boxes indicate the dermal papilla region, shown at higher magnification in the bottom panels. Proliferating cells were quantified by counting PCNA-positive nuclei-colocalized with DAPI within defined regions of interest. Scale bar = 100 μm. ( C ) Quantification of PCNA-positive cells in the dermal papilla of each hair follicle, expressed as the number of PCNA-positive cells per dermal papilla. ( D ) IGF-1 protein levels in whole skin tissue, measured by ELISA and expressed as fold change relative to the saline-treated control group. ( E ) Schematic illustration indicating hair matrix regions analyzed for HMK proliferation. Darker shaded areas represented the analyzed regions (hair matrix). ( F ) Representative immunofluorescence images showing PCNA expression (green) in the hair matrix region of hair follicles from saline- and PDLLA filler-treated mice. Nuclei were counterstained with DAPI (blue). Dashed boxes indicate the hair matrix region, shown at higher magnification in the bottom panels. Quantification was performed by counting PCNA-positive nuclei co-localized with DAPI within standardized regions of interest. Scale bar = 100 μm. ( G ) Quantification of PCNA-positive cells within the hair matrix region, expressed as the number of PCNA-positive cells per hair follicle. ( H ) Schematic illustration indicating hair shaft cortex regions analyzed for keratin expression. Darker shaded areas indicate analyzed regions (hair cortex). ( I ) Representative immunofluorescence images showing the expression of hair shaft cortex-specific hard keratins K35 (type I; green) and K85 (type II; red) in hair follicles from saline- and PDLLA filler-treated mice. Nuclei were counterstained with DAPI (blue). Scale bar = 100 μm. ( J , K ) Quantitative analysis of K35-positive ( J ) and K85-positive ( K ) fluorescence intensity within the hair shaft cortex region, expressed as fold change relative to the saline-treated control group. Data are presented as mean ± standard deviation (n = 5 per group). Group comparisons were performed using the Kruskal–Wallis test and Mann–Whitney U test. **, p < 0.01 vs. Saline. DAPI, 4′,6-diamidino-2-phenylindole; DPCs, dermal papilla cells; ELISA, enzyme-linked immunosorbent assay; HMK, hair matrix keratinocytes; IGF-1, Insulin-like growth factor-1; PCNA, proliferating cell nuclear antigen; PDLLA, poly-D,L-lactic acid.

Journal: International Journal of Molecular Sciences

Article Title: Poly-D,L-Lactic Acid Filler Restores Hair Thickness and Shine by Ameliorating Age-Associated Follicular Decline

doi: 10.3390/ijms27052098

Figure Lengend Snippet: PDLLA filler restores dermal papilla cell proliferation and promotes HMK proliferation in the hair matrix, as well as hair shaft keratin formation, in vivo. ( A ) Schematic illustration indicating the dermal papilla region within the hair follicle, where DPCs are densely localized and were analyzed for proliferation. Darker shaded areas indicate analyzed regions (dermal papilla). ( B ) Representative immunofluorescence images showing PCNA (green) expression in the dermal papilla region of hair follicles from saline- and PDLLA filler-treated mice. Nuclei were counterstained with DAPI (blue). Dashed boxes indicate the dermal papilla region, shown at higher magnification in the bottom panels. Proliferating cells were quantified by counting PCNA-positive nuclei-colocalized with DAPI within defined regions of interest. Scale bar = 100 μm. ( C ) Quantification of PCNA-positive cells in the dermal papilla of each hair follicle, expressed as the number of PCNA-positive cells per dermal papilla. ( D ) IGF-1 protein levels in whole skin tissue, measured by ELISA and expressed as fold change relative to the saline-treated control group. ( E ) Schematic illustration indicating hair matrix regions analyzed for HMK proliferation. Darker shaded areas represented the analyzed regions (hair matrix). ( F ) Representative immunofluorescence images showing PCNA expression (green) in the hair matrix region of hair follicles from saline- and PDLLA filler-treated mice. Nuclei were counterstained with DAPI (blue). Dashed boxes indicate the hair matrix region, shown at higher magnification in the bottom panels. Quantification was performed by counting PCNA-positive nuclei co-localized with DAPI within standardized regions of interest. Scale bar = 100 μm. ( G ) Quantification of PCNA-positive cells within the hair matrix region, expressed as the number of PCNA-positive cells per hair follicle. ( H ) Schematic illustration indicating hair shaft cortex regions analyzed for keratin expression. Darker shaded areas indicate analyzed regions (hair cortex). ( I ) Representative immunofluorescence images showing the expression of hair shaft cortex-specific hard keratins K35 (type I; green) and K85 (type II; red) in hair follicles from saline- and PDLLA filler-treated mice. Nuclei were counterstained with DAPI (blue). Scale bar = 100 μm. ( J , K ) Quantitative analysis of K35-positive ( J ) and K85-positive ( K ) fluorescence intensity within the hair shaft cortex region, expressed as fold change relative to the saline-treated control group. Data are presented as mean ± standard deviation (n = 5 per group). Group comparisons were performed using the Kruskal–Wallis test and Mann–Whitney U test. **, p < 0.01 vs. Saline. DAPI, 4′,6-diamidino-2-phenylindole; DPCs, dermal papilla cells; ELISA, enzyme-linked immunosorbent assay; HMK, hair matrix keratinocytes; IGF-1, Insulin-like growth factor-1; PCNA, proliferating cell nuclear antigen; PDLLA, poly-D,L-lactic acid.

Techniques: In Vivo, Immunofluorescence, Expressing, Saline, Enzyme-linked Immunosorbent Assay, Control, Fluorescence, Standard Deviation, MANN-WHITNEY

G-1 promotes Wnt/Hedgehog-signaling in the human primary DPCs. (A) mRNA expression levels of Wnt signaling-related genes in human hair follicle dermal papilla cells (hDPCs) stimulated with G-1 with or without G-36 measured by qRT-PCR ( n = 6). Internal controls: RPLP0 expression. (B) Activation of the Wnt signaling-related protein β-Catenin in hDPCs stimulated with G-1, with or without G-36, measured using Western blotting ( n = 4). Internal controls: β-actin expression. (C) mRNA expression levels of Hedgehog signaling-related genes in hDPCs stimulated with G-1, with or without G-36, measured using qRT-PCR ( n = 6). Internal controls: RPLP0 expression. (D) Expression levels of Hedgehog signaling-related proteins in hDPCs stimulated with G-1, measured using Western blotting ( n = 8). Internal controls: β-actin expression. * p < 0.05, ** p < 0.01 (Tukey–Kramer's post hoc test). All data are presented as the means ± SE.

Journal: Frontiers in Pharmacology

Article Title: The GPR30 agonist G-1 promotes hair growth via Wnt/Hedgehog signaling in mice

doi: 10.3389/fphar.2025.1570922

Figure Lengend Snippet: G-1 promotes Wnt/Hedgehog-signaling in the human primary DPCs. (A) mRNA expression levels of Wnt signaling-related genes in human hair follicle dermal papilla cells (hDPCs) stimulated with G-1 with or without G-36 measured by qRT-PCR ( n = 6). Internal controls: RPLP0 expression. (B) Activation of the Wnt signaling-related protein β-Catenin in hDPCs stimulated with G-1, with or without G-36, measured using Western blotting ( n = 4). Internal controls: β-actin expression. (C) mRNA expression levels of Hedgehog signaling-related genes in hDPCs stimulated with G-1, with or without G-36, measured using qRT-PCR ( n = 6). Internal controls: RPLP0 expression. (D) Expression levels of Hedgehog signaling-related proteins in hDPCs stimulated with G-1, measured using Western blotting ( n = 8). Internal controls: β-actin expression. * p < 0.05, ** p < 0.01 (Tukey–Kramer's post hoc test). All data are presented as the means ± SE.

Article Snippet: Human hair follicle dermal papilla cells (hDPCs) were obtained from PromoCell (Heidelberg, Germany) and cultured in follicle dermal papilla cell growth medium (Promocell) supplemented with a growth medium Supplement Pack (Promocell).

Techniques: Expressing, Quantitative RT-PCR, Activation Assay, Western Blot

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human dermal papilla cells hdpcs (PromoCell)

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1) Product Images from "A Novel Approach to Pattern Dermal Papilla Spheroids in Dermal–Epidermal Composites Using Non-Adherent Microwell Arrays"

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